The ME803 digital spectrophotometer is a precise instrument used in laboratories for measuring the absorbance and transmittance of light through liquid samples. This device is commonly utilized in fields such as chemistry, biology, environmental science, and material science to analyze the concentration of substances in solution. Here are the detailed specifications and features of the ME803 digital spectrophotometer:

Overview

Spectrophotometer: An analytical instrument used to measure the amount of light that a sample absorbs. It operates by passing a beam of light through a sample and measuring the intensity of light reaching a detector.

Model: ME803 Digital Spectrophotometer

Key Features and Specifications

1. Optical System:
   • Light Source: Tungsten-halogen or deuterium lamp for visible and UV range measurements.
   • Monochromator: High-quality diffraction grating or prism to separate light into its component wavelengths.
   • Wavelength Range: Typically 325 nm to 1000 nm, covering both the UV and visible light spectra.
   • Wavelength Accuracy: ±0.5 nm, ensuring precise selection of the desired wavelength.
   • Spectral Bandwidth: 4 nm, providing adequate resolution for most analytical applications.
2. Measurement Modes:
   • Absorbance (A): Measures the amount of light absorbed by the sample.
   • Transmittance (%T): Measures the amount of light that passes through the sample.
   • Concentration (C): Directly measures the concentration of the substance in the sample (requires calibration with standards).
3. Display and Interface:
   • Digital Display: Large, clear LCD screen showing absorbance, transmittance, and concentration values.
   • Keypad: User-friendly interface for entering commands and parameters.
   • Connectivity: USB or RS-232 ports for data transfer to a computer or printer.
4. Sample Compartment:
   • Cuvette Holder: Accommodates standard 10 mm path length cuvettes; can hold multiple cuvettes for batch analysis.
   • Adjustable Holder: Allows the use of different cuvette sizes and test tubes.
5. Data Handling:
   • Internal Memory: Stores a significant number of readings and calibration data.
   • Software Compatibility: Can be connected to external software for data analysis and reporting.
6. Calibration and Standards:
   • Automatic Calibration: Built-in calibration routines using known standards.
   • Standard Curve Creation: Allows the creation of calibration curves for various analytes.
7. Power and Dimensions:
   • Power Supply: 220V/50Hz or 110V/60Hz, with appropriate adaptors.
   • Dimensions: Compact design for bench-top use, typically around 450 mm x 350 mm x 200 mm (L x W x H).
   • Weight: Approximately 10 kg, making it portable within the laboratory.

Applications

1. Chemical Analysis:
   • Quantifying the concentration of substances in solution.
   • Measuring reaction kinetics and monitoring chemical reactions.
2. Biological and Medical Research:
   • Analyzing nucleic acids, proteins, and other biomolecules.
   • Assessing the purity of samples.
3. Environmental Science:
   • Monitoring water quality by measuring pollutant concentrations.
   • Analyzing soil and plant extracts.
4. Industrial Applications:
   • Quality control in manufacturing processes.
   • Testing raw materials and finished products.

Advantages

1. High Precision: Accurate measurements due to high wavelength accuracy and stability.
2. Versatility: Suitable for a wide range of applications in various scientific fields.
3. User-Friendly: Easy-to-use interface and clear digital display.
4. Data Management: Efficient storage and transfer of measurement data for analysis and reporting.
5. Compact Design: Occupies minimal bench space and is easy to transport.

Care and Maintenance

1. Cleaning:
   • Regularly clean the cuvette holder and optical path with a lint-free cloth and appropriate solvents.
   • Avoid using abrasive materials that could scratch the optical components.
2. Calibration:
   • Perform regular calibration checks using standard solutions to ensure accuracy.
   • Follow manufacturer guidelines for wavelength and absorbance calibration.
3. Storage:
   • Store in a dry, dust-free environment when not in use.
   • Cover the instrument to protect it from dust and contaminants.

Environmental Considerations

1. Energy Efficiency: Designed to minimize power consumption during operation.
2. Material Recycling: Many parts can be recycled according to local regulations at the end of the instrument’s life cycle.
3. Sustainable Use: Use of durable materials to ensure long-term use and reduce the need for frequent replacements.

Conclusion

The ME803 digital spectrophotometer is a reliable and versatile instrument for measuring the absorbance and transmittance of light in various samples. Its high precision, user-friendly interface, and robust design make it an essential tool in any laboratory. Proper care and maintenance will ensure its longevity and consistent performance, making it a valuable asset for scientific research and industrial applications.